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- Keywords Real Time PCR;virus;Inactivation and removal efficiency; 实时荧光定量PCR;病毒;灭活去除效率;
- Keywords Real time PCR;Rumen microorganisms;Quantification;Plant oil;Methane; 瘤胃微生物;定量;植物油;甲烷;
- Use RT-PCR and real time PCR to quantify the HLA-G mRNA on deciduas. RT-PCR和real time PCR法检测胎盘蜕膜组织HLA-G mRNA。
- Keywords hepatitis B virus;real time PCR;HBeAg; 乙型肝炎病毒;聚合酶链反应;荧光定量;
- The plasma biochemical indexes were detected by using the kit, and real time PCR was used to analyze the expression of adipogenesis genes with time series. 试剂盒检测血脂生化指标，荧光定量 PCR 技术检测聚脂相关基因的转录表达规律。
- Totally 240 throat swab specimens collected from adults with flu like symptoms were detected both by the multiplex real time PCR system and conventional virus isolation. 我们收集240名成人具有类流感症状的咽喉拭子检体，利用此技术进行病毒筛检，并与病毒培养结果比较。
- Keywords real time quantitative PCR;Roundup Ready soybean;GMO detection; 荧光定量PCR;转基因大豆;转基因检测;
- The population of M. formicicum by real time PCR was lower than the population of M. formicicum by the method of most probable number, and the positive correlation between them was very high(r=0.957. P<0.01). M.;formicicum采用Real Time PCR方法测得的数量低于采用传统最大或然数法测得的数量;但两者之间具有很高的相关性(r=0
- Methods A549 cells were treated with different cytotoxic drugs for 24hours. The changes of APE/ref-1 expression in A549 after drug-treatment were detected by real time PCR, western blot and streptavidin peroxidase immunohistochemistry staining. 方法 通过免疫组化染色明确APE/ref-1在A549细胞中的表达后,以特定浓度的临床常用细胞毒性药物对A549细胞进行诱导,采用实时荧光定量PCR、Western Blot和免疫组化技术检测药物诱导前后A549细胞内APE/ref-1表达的变化情况。
- Genehunter analysis is concordant with ETDT. By real time PCR, OAZ(OLF1/ EBF associated zinc finger protein)gene which is located in the positive locus showed higher expression in SLE patients than in normal controls. 对候选基因的研究显示 OAZ(OL F1/ EBF- associated zinc finger protein)基因表达显著高于正常对照。
- Methods Quantitative detection of T cell receptor excision DNA circles (TRECs) in DNA of peripheral blood mononuclear cells from 8 cases with CML, CD4 + or CD8 + cells from 3 cases with CML were preformed by real time PCR (TaqMan) analysis. 方法 利用实时定量PCR(Taq Man)方法检测 8例CML患者外周血单个核细胞、3例CML患者CD4 + 和CD8+ 细胞中TRECs的水平。
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 应用脂质体Lipofectamine2000转染人结肠癌LoVo细胞,转染成功后以G418筛选4周,实时荧光定量RT-PCR(RealTimeRT-PCR)检测cerb-B1基因的mRNA表达,Western-blot检测EGFR蛋白的表达。
- RT-PCR, real time PCR or NASBA are used to pathogen test on cloacal or throat swab and wild birds.If the conformity rate >70% for HI antibody, it can be certified as conformity of vaccination. 疫情监测用RT-PCR或荧光定量PCR或NASBA(以来核酸序列的扩增技术)等方法进行泄殖腔拭子、咽喉拭子和野鸟粪便的病原学检测。
- In order to to detect Penicillium expansum rapidly in apple juice concentration,the real time PCR method was used to detect Penicillium expansum and the conditions were also optimized. 为了研究苹果汁生产中棒曲霉素产生菌的快速检测,利用实时PCR方法快速检测扩展青霉的可能性,对扩增条件进行优化。
- Lee, D. S., “Reserch and Development of a Novel Real Time PCR Machine,” Department of Mechanical Engineering, National Taiwan University, Dissertation for Doctor of Philosophy, 2004. 吴孟勋，新型复式即时定量聚合酶连锁反应检测机台开发与研究，国立台湾大学机械工程研究所硕士论文，台北，2004。
- Keywords Real Time Quantitative PCR;chronic myelogenous leukemia;imatinib mesylate;BCR-ABL fusion gene;resistance mechanism; 实时定量PCR;慢性髓细胞白血病;伊马替尼;BCR-ABL融合基因;耐药机制;
- Real time PCR quantification methods 实时定量法
- Keywords Real Time Correction;Geometric Error;Anti-Abbe Construction; 关键词实时修正;几何误差;违阿贝结构;
- Keywords Real time FTIR;Infrared laser;Cationic cyanine dye borate complex; 实时红外光谱分析;红外激光;阳离子菁染料-有机硼络合物;
- Keywords PCR;real time PCR;retroviral vector;gene transfection;clonal PCR;linear amplification mediated PCR; 实时PCR;逆转录病毒载体;基因转染;克隆PCR;线性扩增介导的PCR;
